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How To Use the Iron Contaminated pH Machine

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How To Use the Non-Iron Contaminated pH Machine
(Room 403)
1. Press "On/Off" to turn machine on. 2. Press "cal" (calibrate).
3. Calibrate for first pH reading:
a. If pHing on the acid side of the scale, open pH 4 calibration vial (pink). b. If pHing on the basic side of the scale, open pH 7 calibration vial (yellow).
4. Rinse off the electrode into the Non-Fe contaminated waste beaker with the squeeze bottle of Milli-Q water. 5. Blot electrode end with Kim-wipe.
6. Place rinsed electrode in the calibration vial. 7. Wait for machine to stop changing numbers and a small "4" (or "7") in a box will appear next to "calibrate" at the top of the screen.
8. Rinse electrode. 9. Blot.
10. Machine will automatically begin to calibrate the second value.
a. If pHing on the acid side of the scale, open pH 7 calibration vial (yellow). b. If pHing on the basic side of the scale, open pH 10 calibration vial (blue).
11. Place electrode into second calibration vial. 12. Once the second value is calibrated, a small "7" (or "10") in a box will appear next to the first calibrated value.
13. The screen will briefly display a % slope value. a. If the value is less than 90.0% or more than 105% then the electrode is not working properly.
b. Replace electrode. 14. Rinse electrode. 15. Place electrode in a non-iron contaminated electrode holder-arm and lower into solution.
16. Press "read."
17. Machine will display "ready" next to pH value when it has measured a stable pH. 18. Once your solution is at the desired pH
19. Rinse electrode with Milli-Q
20. Blot electrode
21. Store electrode in pH 4 vial marked "Store Electrode Do Not Use To Calibrate."
22. Press "On/Off" again to turn machine off.
Radik, Melanie 1-1
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